In modern life, flame retardants are almost everywhere. Thousands of flame retardant molecules are just like the alert little guards, and they are silently guarding your life and property safety. Let us stay away from the threat of fire. However, flame retardants may become a chronic poison in our lives.
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) is a hydrogen ion buffer which has a good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. Recently, HEPES can also as a composition of pain control agents, and reversing demyelinating damage.
MOPS, 3-(N-Morpholino)propanesulfonic acid, is an important biological buffer, the buffer range is 6.5~7.9; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is also a hydrogen ion buffer which has a Good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. They are both widely used in biological and chemical experiments. When the two combine to act as a gamete buffer, what are the advantages?
Standard Test Method for Curing Properties of Extruded Resins by Thermal Analysis
Original standard name: standard test method for testing the curability of extruded resin by thermal analysis using thermal analyzer sample pan
Guanidine hydrochloride is often used as an intermediate in medicines, pesticides, dyes and other organic compounds, it is also an important raw material for sulfonamides and folic acid; it can be used as a strong denaturants in the extraction of total RNA from cells. The proportion of traditional refined guanidine hydrochloride synthetic preparation process is simple, and the operation in each process is not strict: such as mixing impurities during stirring, temperature is difficult to observe, and weighing is inaccurate. Both of them lead to the problem that the low purity of the Guanidine HCL and the incomplete reaction. Then how to solve these problems?
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) is a hydrogen ion buffer which has a good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. The commonly used concentration is 10~50mmol/L, the general culture solution contains 20mmol/L HEPES can have a good buffer capacity. Then how to prepare it?
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) is a hydrogen ion buffer which has a good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. It can be used in a wide range of biochemical reactions, such as, in the research of organelles and highly denatured pH-sensitive proteins and enzymes, as well as biochemical diagnostic kit, DNA/RNA extraction kits and PCR diagnostic kit. Below we will list the common used recipes.
Tris(Hydroxymethyl) Aminomethane (TRIS) buffer solution is a biological reagent, which is a buffer solution commonly used in biological preparation and medical preparation. For example, it’s commonly used in peprotech recombinant cytokines and protein solubilization, and widely used as solvent for nucleic acid and proteins electrophoresis. Usually, it is used as a liquid, but it is not easy to store for a long time and the preparation is inconvenient. If it is made into a tablet, the above problem will be well solved.
Good's buffers are zwitterionic buffers, which are generally sulfamic acid-based organic compounds, their pH values are relatively stable and does not react with various enzymes and chemical reagents. It is mainly used in the field of biology and medicine. CAPS (N-Cyclohexyl-3-Aminopropanesulfonic Acid) is one of the commonly used Good's buffers in separation of basic drugs by enzymatic chemistry and HPLC. Therefore, its preparation is very important. In order to reduce the reaction conditions, it is generally carried out under the conditions of a catalyst and infrared light irradiation in the production process, while the production cost is increased. And at the same time, the purity of the preparation is difficult to be improved due to the instability of the reaction conditions. This article describes a low-cost method for preparing high purity CAPS.
1. The main technical and economic indexes of process selection include: treatment of unit water investment, reduction of unit pollution investment, treatment of unit water consumption and cost, reduction of unit pollutant power consumption and cost, occupation of land, operation performance reliability, management and maintenance of the degree of difficulty, overall environmental benefits and so on.
MOPS,3-(N-Morpholino)propanesulfonic acid, is an important biological buffer, the buffer range is 6.5~7.9, which is widely used in biological and chemical experiments, such as electron transfer and phosphorylation in chloroplast thin layer, culture of Streptomyces coelicolus and the production of cephalosporins, and electrolyte system component in isoelectric focusing electrophoresis (IEF) of two-dimensional gel electrophoresis. So the preparation of MOPS Buffer is very important.
In biochemical experiments,the abbreviation “CAPS” is appeared frequently,and it has two completely different meanings.
In biochemical experiments, the buffer recipe is often the top priority of the experiment, and a truly reliable recipe can definitely change the fate of the experiment.
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) is a hydrogen ion buffer which has a good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. How to synthesize HEPES with high purity?
Guanidine hydrochloride is often used as an intermediate in medicines, pesticides, dyes and other organic compounds, it is also an important raw material for sulfonamides and folic acid; it can be used as a strong denaturants in the extraction of total RNA from cells, and used for denaturation and complexation of proteins. It can be used as an antistatic agent for synthetic fibers. In this article, we will introduce you another application of guanidine hydrochloride——to improve the property of gold nanoclusters（AuNCs）.
Thermal analysis technology is a group of technologies to characterize the relationship between the properties of materials and temperature. It is widely used in qualitative and quantitative characterization of thermal, physical, mechanical and stability properties of materials, and has very important practical significance for the research and development of materials and quality control in production.At present, thermal analysis technology is increasingly becoming one of the indispensable and important means in the research, development and quality control of rubber materials.
The zwitterionic buffer, also known as Good's Buffer, is a series of N-substituted sulfamic acids that have good pH stability and are inert to a variety of chemicals and enzymes. Among them, the morpholinic buffers, including MOPS Sodium Salt, MES, and MOPSO, have some common character, for example, they do not form complexes with most metal ions and are therefore suitable for solutions containing metal ions. But what is the difference between them?
